A home made kit for plasmid DNA mini-preparation

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منابع مشابه

Preparation and Expression of M2 Gene Plasmid DNA for Potential use in Influenza A Vaccine Production

No abstract this article: Preparation and Expression of M2 Gene Plasmid DNA for Potential use in Influenza A Vaccine Production

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Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA.

A mini-F-coded protein, named F2 protein, binds specifically to mini-F DNA. This protein has a molecular weight of 37,000 and is coded by the A2 segment of the mini-F genome (47.3 to 49.4 kilobases on the F coordinate map). The binding site is located also in the A2 segment of mini-F. This binding site is lost by spontaneous deletion when the A2 segment alone, but not A2 together with its neigh...

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Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine

Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity. Objective: To evaluate the use of a DNA immunization strategy for protection against anthrax, a plasmid was constructed. Methods: The partialsequence of protective antigen of Bacillus anthracis, amino acids 175-764, as a potent immunogenic targe...

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ژورنال

عنوان ژورنال: African Journal of Biotechnology

سال: 2003

ISSN: 1684-5315

DOI: 10.5897/ajb2003.000-1018